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Circular Oligonucleotide Synthesis Services

Over the last decade, hundreds of oligonucleotide analogs have been designed with the aim of improving their properties for a variety of applications. Circular oligonucleotides, or circular DNA or circular RNA oligonucleotides designed to undergo triplex formation with single-stranded DNA and RNA targets, have attracted increased attention because they are more resistant to nucleases than their linear counterparts, showing unique DNA recognition properties and are efficient templates for DNA and RNA polymerases. Many of these circular oligonucleotides bind with greater affinity, sequence selectivity and are more resistant to nucleases compared to their linear counterparts. They also have interesting properties in several diagnostic and therapeutic applications, and they have been used in the study of noncanonical DNA structural motifs[2].

Circularized single-stranded deoxyribonucleotides (ssDNAs) are more resistant to nuclease activity than their linear counterparts, a property that may be important for in vivo studies. Circular DNAs have been investigated for their unique DNA binding properties and also as useful models in studying DNA structures such as hairpin motifs by NMR. DNA circles are also currently being used for more diagnostic applications, such as the creation of padlock probes. Circular oligonucleotide templates have also been used for the synthesis of concatemeric polypeptides. Additionally, circular ssDNAs offer a novel means of both DNA and RNA amplification as these molecules are accepted as templates by both DNA and RNA polymerases[3].

Synthetic Methods for Circular Oligonucleotides

Methods for the synthesis of circular DNAs and RNAs have been advanced as the need for these structures increased. Several published studies have focused on nontemplated cyclization of short synthetic oligonucleotides. Both solid-phrase and liquid-phrase methods have been investigated.

  1. Solid-phase procedures are almost universally considered to be superior in terms of speed and effectiveness, and they also provide the ability to simulate pseudodilution conditions for the crucial cyclization step. Different solutions have been proposed for the cyclization of immobilized linear precursors[1].
  2. Circular Oligonucleotide Synthesis ServicesCircular Oligonucleotide Synthesis. Anchoring the 3'-end nucleotide to the solid support.[2]

  3. Major issues of solid-phase synthesis that need to be addressed are scale-up and manufacturing cost. Liquid-phase approaches may overcome the limitations. Behind the progress of solid-phase synthesis, researchers have investigated unique liquid-phase approaches. Among them, several methods utilizing soluble supports instead of solid resins combine the advantages of solid-phase synthesis and liquid-phase synthesis, and are expected to be used in industrial applications [2].

Our Services:

We offer high-quality cyclic oligonucleotide synthesis services. These synthetic cyclic DNA or cyclic RNA oligonucleotides synthesized by liquid- and solid-phase synthesis procedures are purified using denaturing polyacrylamide gel electrophoresis (PAGE) with >95% purity.


  • Length- 35 to 200 mers
  • Quantities- 100, 200, 500 pmole
  • Purification- HPLC or PAGE purified
  • Validation- MS and PAGE analysis plus additional QA procedures

Competitive Advantages:

  • Flexible synthesis scales
  • Bulk orders are available upon request
  • Stringent quality control
  • High-quality and cost-effective
  • Fast turnaround: 5-10 business days


  • Synthetic RNA/DNA lyophilized powder
  • COA
  • Test report (MS & HPLC)


  1. A. M. Diegelman, E. Kool. Chemical and enzymatic methods for preparing circular single-stranded DNAs. Current Protocols in Nucleic Acid Chemistry. 5.2.1-5.2.27.(2000)
  2. Oligonucleotide synthesis: methods and applications / edited by Piet Herdewijn. (Methods in molecular biology ; 288)
  3. Lesca M. Holdt, Alexander Kohlmaier, Daniel Teupser. Circular RNAs as Therapeutic Agents and Targets. Front. Physiol. 9:1262. (2018)

*For Research Use Only. Not for use in diagnostic procedures.

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