Nucleic Acid-Oligo Bioconjugation Services
Although oligonucleotides have been considered as a new drug for more than three decades, their therapeutic application has not fully met expectations to date. All nucleic acid drugs have poor pharmacokinetics, lack cell membrane permeability, and are often unstable and even sometimes produce off-target effects. To realize the full potential of oligonucleotide therapy, the use of conjugated oligonucleotides is an effective approach. These conjugates are lipophilic or targeting in nature. Their main functions are to enhance targeting, promote vectorization, reduce the sensitivity of oligonucleotides to nuclease activity, eliminate or reduce the need for toxic transfection agents, and generally improve pharmacological activity and efficacy[1].
Bioconjugated oligonucleotides, including novel conjugates with peptides, proteins, carbohydrates, aptamers, small molecules and GalNAc, provide enhanced tissue targeting, cell internalization, endosomal escape, target binding specificity, resistance to nucleases, etc[2].
Novel bioconjugated oligonucleotides[1]
Our Oligo Bioconjugation Services
With our advanced synthesizers and over a decade of experience in nucleic acid drug development, we can provide custom oligonucleotide conjugation services. We have the most efficient and robust oligonucleotide conjugation technology, allowing single and double-stranded oligonucleotides to be conjugated to the molecule of your choice. Our most friendly and efficient oligonucleotide labeling kits enable the perfect combination of oligonucleotides with antibodies, enzymes, streptavidin, avidin, fluorescent proteins, biotin, etc. In addition, we can also provide reliable custom oligonucleotide conjugation services to meet your application needs for assays, antibody production, therapeutic research and more.
Quality Assurance
During synthesis, each oligonucleotide conjugate is rigorously monitored according to strict biosynthetic quality assurance and quality control standards. The final product is identified by mass spectrometry, and its high purity is ensured by high-throughput gel electrophoresis, high-performance liquid chromatography or other appropriate QC methods.
The types of bioconjugation include, but are not limited to:
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Oligo Fluorescent Labeling
Fluorescent-labeled oligonucleotide probes provide the advantages of the highest sensitivity and specificity paired with low toxicity.
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Peptide-oligonucleotide Conjugation
Each peptide-oligonucleotide is meticulously monitored at each production cycle according to stringent quality assurance and quality control standards.
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Antisense Oligo Conjugation
We ensure that the antisense oligo bioconjugates are of the highest quality and yield.
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Oligo-Antibody Conjugation
All antibody oligonucleotide conjugates are analyzed by SDS-PAGE with less than 10% of unconjugated antibodies.
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Oligo-Enzyme Conjugation
Oligo-enzyme conjugation offers the best overall performance with the highest sensitivity, specificity, simplicity and rapid detection.
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Oligo-Carrier Conjugation
Custom nucleic acid-carrier conjugation services, using single or double-stranded DNA or cDNA for anti-DNA antibody generation.
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Carbohydrate-Oligonucleotide Conjugation
Oligonucleotide-carbohydrate conjugates are used in antisense biotechnology and in the study of glycosylated DNA functioning in vitro.
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PEG Conjugated Oligonucleotide
PEGylation, the attachment of poly(ethylene glycol) (PEG), has been adopted to improve the pharmacokinetic properties of oligonucleotide therapeutics.
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Gold Nanoparticles labeled Oligonucleotide
Gold nanoparticles (AuNPs) can be readily synthesized and promptly bioconjugated with similarly sized oligonucleotides for biodetection.
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Biotin Labeled Oligonucleotides
Biotinylated oligonucleotides can be used to attach specifically to streptavidin-enzyme-conjugates, streptavidin-protein-conjugates, streptavidin coated surfaces or streptavidin-dye-conjugates.
Advantages:
- Oligos-Nucleic Acid Bioconjugation
- Quality oligo synthesis
- Flexible bioconjugation chemistries
- A wide choice of target molecules
- Differenet modifications available
- High yield
- High purity
- Various QC methods
- Micrograms to grams-scale
Delivery Specifications:
- Lyophilized sample
- COA
- MS/HPLC report
Since its establishment, Creative Biolabs has developed quality products with advanced technologies, and has made considerable progress in the development of oligonucleotide drugs, large-scale production of oligonucleotides, raw material drug quality research, GMP production line construction and GMP management. You can contact us for any oligonucleotide drug research and development needs.
References
- Sebastien Benizri.Bioconjugated Oligonucleotides: Recent Developments and Therapeutic Applications. Bioconjug Chem. 2019 Feb 20; 30(2): 366-383.
- Winkler J. Oligonucleotide conjugates for therapeutic applications. Ther Deliv. 2013;4(7):791-809.
*For Research Use Only. Not for use in diagnostic procedures.