Oligonucleotide Fluorescent-labeling Service
Radiolabeling and fluorescent labeling have found wide application in DNA and RNA hybridization and sequence analysis. Fluorescent dyes conjugated with nucleic acids offer some essential advantages over a radioactive label. Fluorescent dyes can be detected in real-time with high resolution and several dyes can be monitored in one experiment. Fluorescent dyes are free of radiation hazards and the consequent problems of waste handling and disposal.
Fluorescent dyes conjugating with oligonucleotides have become increasingly important in biophysical studies of the structure, function, and dynamics of nucleic acids. These studies require the site-specific and stable incorporation of fluorescent tags into the system of interest. Oligonucleotides (RNA/DNA) do not contain naturally occurring fluorophores, therefore, to perform fluorescence assays, it is necessary to introduce these groups. For short oligonucleotide constructs, a fluorophore may be conjugated to a nucleotide at the 5' end, 3' end or internally. For longer oligonucleotide constructs, a fluorophore label may be added by ligation or hybridization of short fluorescently labeled oligonucleotides.
Strategies for labeling nucleic acids based on solid-phase synthesis (Nils Klöcker, etc.)
Our Fluorescent Labeling Service
Based on our advanced synthesizer, mature technology and extensive and successful project experience, we can provide a one-stop oligo fluorescent labeling service from design to delivery for your needs.
We use two fluorescent labeling methods:
1. Direct oligosaccharide labeling with cyanoethyl phosphoramide-containing derivative dyes can be used for 5', internal standard and 3' labeling in the chemical synthesis process.
2. The introduction of active amino or thiol groups into synthesized oligonucleotides provides acceptors for subsequent chemical fluorescent labeling.
A variety of fluorescent probes:
| Fluorescein | Coumarin | BODIPY | Cascade Blue |
| Lucifer Yellow | Phycobiliprotein Derivatives | Cyanine Dye Derivative | Chelates for Time-resolved fluorescent |
| Quantum Dot | Rhodamine |
Purification:
The final product is identified by mass spectrometry and purified by high-performance liquid chromatography or other appropriate QC methods to remove mutation products and unlabeled oligosaccharides. During synthesis, each oligonucleotide conjugate is rigorously monitored according to strict biosynthetic quality assurance and quality control standards.
Advantages:
- High success rate
- Quality oligos synthesis
- A variety of fluorescent probes
- High yield
- High purity
- Guaranteed Quality: various QC measures
- Micrograms to grams-scale
- A good after-sales service
Delivery Specifications:
- Lyophilized sample
- COA
- MS/HPLC report
Since its establishment, Creative Biolabs has made considerable progress in the development of oligonucleotide drugs, large-scale production of oligonucleotides, raw material drug quality research, GMP production line construction and GMP management. You can contact us for any oligonucleotide drug research and development needs.
References
- Dmitri Proudnikov,Chemical methods of DNA and RNA fluorescent labeling. Nucleic Acids Research, 1996, Vol. 24, No. 22 4535-4532
- Sharla Wood, Fluorescence Labeling of Nucleic Acids. Encyclopedia of Biophysics, 2013
*For Research Use Only. Not for use in diagnostic procedures.