Peptide-oligonucleotide conjugation Service
Relying on advanced synthesizers, rich project experience and strong PhD think tank, we can provide high-quality peptide-oligonucleotide conjugation (POCs) services.
Introduction of POCs
Oligonucleotides are a type of short-chain nucleotides, which have strong biological activity. Selected by exponential enrichment ligand evolution (SELEX) technology, oligonucleotides can specifically bind to proteins, small molecules, ions, cells, and other receptors with strong specificity and high sensitivity, and can be used for various biosensing applications. Also, oligonucleotides can be used as gene therapy drugs or drug precursors. However, oligonucleotides have the disadvantages of poor cell permeability, easy decomposition and low affinity to the target. In contrast, peptides have small molecular weight, adjustable charge and resistance to enzymatic hydrolysis, etc. Coupling the peptide and the oligonucleotide can improve the cell permeability and the biological activity of oligonucleotides.
Structure of peptide-oligonucleotide conjugates[1]
In recent years, the development of various peptide screening technologies, especially phage display technology, has led to the emergence of a large number of peptides with functions of identifying, detecting and treating cancer cells. The discovery of these peptides has brought new ideas and methods for the diagnosis and treatment of various cancers. By effectively linking peptides with oligonucleotides, we can obtain biomolecules with dual functions or drugs with synergistic therapeutic effects that can achieve integrated diagnosis and treatment. Therefore, peptide-oligonucleotide couplings have high practical applications in biosensing and cancer therapy.
A nucleic acids-peptides conjugate construction approach[2]
Our Peptide-oligonucleotide Conjugation Service
We have the most efficient and robust oligonucleotide conjugation technology allowing single and double-stranded oligonucleotides to be conjugated to the peptide of your choice. In addition, we can also provide reliable custom oligonucleotide conjugation services to meet your application needs for assays, antibody production, therapeutic research and more.
The types of bioconjugation service include, but are not limited to:
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DNA-Peptide Conjugation Service
We provide a wide variety of synthesis scales, modifications, treatments and purification grades to customize your conjugates according to your requirements.
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RNA-Peptide Conjugation Service
Each peptide-RNA hybrid is meticulously monitored at each production cycle according to stringent quality assurance and quality control standards.
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siRNA-Peptide Conjugation
Peptide-siRNA complexes are produced in our state-of-the-art chemical biology facilities and through expertise in cross-linking chemistries.
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Quality Assurance
During synthesis, each POC conjugate is rigorously monitored according to strict biosynthetic quality assurance and quality control standards. The final product is HPLC-purified and QC-tested by Mass Spectrometry.
Advantages:
- Quality oligos synthesis
- Different modifications available
- High yield
- High purity
- Various QC methods
- Up to 20 mg
- Length of DNA, RNA, siRNA conjugates of 10 - 40 bases
- Peptide lengths of 5-22 amino acids
Modifications (If any modification you are looking for is not on the list, please contact us.):
Oligo |
Peptide |
| Phosphorothioate linkage | Acetylation |
| Dye labeling (FAM, HEX ,TET, etc) | Phosphorylation |
| Biotinylation | methylation |
| Spacer (C3, HEG, etc) | Biotinylation |
| 2'-O-methyl RNA | Spacer (Ahx, AEEAc, etc) |
| Fatty acids conjugation | |
| Dye-Labeling (FAM, TAMRA, etc) |
Delivery Specifications:
- Lyophilized sample
- COA
- MS/HPLC report
Since its establishment, Creative Biolabs has made considerable progress in the development of oligonucleotide drugs, large-scale production of oligonucleotides, raw material drug quality research, GMP production line construction and GMP management. You can contact us for any oligonucleotide drug research and development needs.
References
- Tara MacCulloch,Emerging applications of peptide–oligonucleotide conjugates: bioactive scaffolds, self-assembling systems, and hybrid nanomaterials. Org. Biomol. Chem., 2019, 17, 1668-1682
- Synthesis of aldehyde-linked nucleotides and DNA and their bioconjugations with lysine and peptides through reductive amination.Chem. Eur. J. 2012, 18, 4080-4087.
*For Research Use Only. Not for use in diagnostic procedures.