RNA-Peptide Conjugation Service
Modifications that can be easily applied to RNA can include isotopic labeling, fluorescent or other molecular probes, proteins, peptides, lipids, glycosides or nucleic acid conjugates that can be introduced using combinations of synthetic chemistry, enzymatic incorporation, and various combinations of conjugation chemistries. These labels, conjugations and ligations with RNA are key to further research and application of RNA because they make it possible to visualize, elucidate the structure, locate and biodistribute the modified RNA[1].
RNA-peptide conjugates, a type of conjugations with RNA, have been constructed for versatile therapeutic application, such as the delivery of siRNA or the screening of a library of peptides in a mRNA display method and can be exploited in a wide range of applications from nanotechnology to drug delivery and antisense technology. Efficient reaction for the formation of covalent linkage between RNA and peptide is important for developing practical applications. Many methods have been reported to conjugate an oligonucleotide (RNA or DNA) with an oligopeptide by applying various chemistries. Among them, a new method for the construction of receptor-based fluorescent sensors and catalysts using the ribonucleopeptide (RNP) as a scaffold[2].
Schematic illustration of the construction of the covalently-linked fluorescent ribonucleopeptide (RNP) sensor [1]
Our RNA-Peptide Conjugation Services
With our advanced synthesizers and over a decade of experience in nucleic acid drug development, Creative Biolabs can provide custom oligonucleotide conjugation services. We provide reliable customized RNA-peptide conjugates for a variety of biological applications, such as cell screening, antibody production, therapeutic research and in vivo research and more. For RNA peptide conjugates, 2'-O-methyl RNA is recommended to prevent ribonucleases degradation of RNA-peptide conjugates.
Quality Assurance
During synthesis, each RNA-peptide conjugate undergoes strict quality control, identified by mass spectrometry, and its high purity is ensured by high throughput gel electrophoresis, high-performance liquid chromatography or other appropriate QC methods.
Modifications (If any modification you are looking for is not on the list, please contact us.):
Oligo |
Peptide |
| Phosphorothioate linkage | Acetylation |
| Dye labeling (FAM, HEX ,TET, etc) | Phosphorylation |
| Biotinylation | methylation |
| Spacer (C3, HEG, etc) | Biotinylation |
| 2'-O-methyl RNA | Spacer (Ahx, AEEAc, etc) |
| Fatty acids conjugation | |
| Dye-Labeling (FAM, TAMRA, etc) |
Advantages:
- Oligos Nucleic Acid Bioconjugation
- Quality oligo synthesis
- Flexible bioconjugation chemistries
- A wide choice of target molecules
- Different modifications available
- High yield
- High purity
- Various QC methods
- Micrograms to grams-scale
- Length of RNA, siRNA conjugates: 10 - 40 bases
- Peptide lengths: 5-22 amino acids
Delivery Specifications:
- Lyophilized sample
- COA
- MS/HPLC report
Since its establishment, Creative Biolabs has developed products with advanced technologies, and has made considerable progress in the development of oligonucleotide drugs, large-scale production of oligonucleotides, raw material drug quality research, GMP production line construction and GMP management. You can contact us for any oligonucleotide drug research and development needs.
References
- Eduardo Paredes. RNA labeling, conjugation and ligation.2011 Jun;54(2):251-9.
- Shun Nakano, Taiki Seko, Zhengxiao Zhang and Takashi Morii. RNA -Peptide Conjugation through an Efficient Covalent Bond Formation. Appl. Sci. 2020, 10, 8920
*For Research Use Only. Not for use in diagnostic procedures.