DNA-Peptide Conjugation Service
Oligonucleotides have extremely important applications in disease treatment and gene function research, but their ability to antagonize nucleases and penetrate cell membranes is weak, which limits their application. Many polypeptides have high cell permeability. If polypeptides and oligonucleotides are combined via covalent bonds to form conjugates, the permeability of the oligonucleotides to cells and the antagonism of nucleases can be improved.
Peptide–oligonucleotide conjugates (POCs) are covalent constructs that link a molecule like DNA to synthetic peptide sequences. These materials merge the programmable self-assembly of oligonucleotides with the bioactivity and chemical diversity of polypeptides. In recent years, the widespread use of POCs has been seen in a range of fields, driven by the relative advantages of each molecular type[1].
The synthetic methods of peptide-oligonucleotide conjugates mainly include liquid-phase synthesis and solid-phase synthesis, but there are many ways to conjugate the molecules. The commonly used conjugation methods are mainly oxime bond, amide bond, disulfide bond, phosphorus linked by acyl bond, hydrazone bond, ureide bond, etc.
Several criteria for the oligonucleotide-peptide conjugation method are used for evaluation, such as easy derivatization of incoming oligonucleotides and peptides, high efficiency of the conjugation reaction, good stability of the conjugates, and satisfactory conjugate performance[2].
DNA-peptide conjugation reaction[2]
Our DNA-Oligo Bioconjugation Services
With our advanced synthesizers and over a decade of experience in nucleic acid drug development, we can provide a wide variety of synthesis scales, modifications, treatments and purifications to customize your conjugates according to your requirements.
As experts in bioconjugation, we use a variety of cross-linking chemistry, including (but not limited to) natural linking chemistry and click chemistry. In addition, we can also provide reliable custom DNA-Oligo conjugates to meet your application needs for assays, antibody production, therapeutic research and more.
Quality Assurance
During synthesis, each DNA-peptide conjugate is rigorously monitored according to strict biosynthetic quality assurance and quality control standards. Each POC is HPLC-purified and QC-tested by Mass Spectrometry.
Modifications (If the modification you are looking for is not on the list, please contact us.):
Oligo |
Peptide |
| Phosphorothioate linkage | Acetylation |
| Dye labeling (FAM, HEX ,TET, etc) | Phosphorylation |
| Biotinylation | methylation |
| Spacer (C3, HEG, etc) | Biotinylation |
| Spacer (Ahx, AEEAc, etc) | |
| Fatty acids conjugation | |
| Dye-Labeling (FAM, TAMRA, etc) |
Advantages:
- Quality oligo synthesis
- Different modifications available
- Cross-linking chemistries
- High yield
- High purity
- Various QC methods
- Up to 20 grams
- A wide range of synthesis scales
- 10 - 40 bases length of DNA conjugates
- Peptide lengths of 5-22 amino acids
Delivery Specifications:
- Lyophilized sample
- COA
- MS/HPLC report
Since its establishment, Creative Biolabs has developed quality products with advanced technologies, and has made considerable progress in the development of oligonucleotide drugs, large-scale production of oligonucleotides, raw material drug quality research, GMP production line construction and GMP management. You can contact us for any oligonucleotides drug research and development needs.
References
- Tara MacCulloch,Emerging applications of peptide–oligonucleotide conjugates: bioactive scaffolds, self-assembling systems, and hybrid nanomaterials. Org. Biomol. Chem., 2019, 17, 1668-1682
- Igor A. Kozlov, Peter C. Melnyk, John P. Hachmann, David L. Barker, Michal Lebl, and Chanfeng Zhao. EVALUATION OF DIFFERENT CHEMICAL STRATEGIES FOR CONJUGATION OF OLIGONUCLEOTIDES TO PEPTIDES. Nucleosides, Nucleotides, and Nucleic Acids, 26:1353-1357, 2007
*For Research Use Only. Not for use in diagnostic procedures.