Custom Phosphoramidites and Support Services
Synthetic oligonucleotides are indispensable in many different fields. Millions of oligonucleotides are synthesized every day for use in research laboratories, hospitals, and industries. With the development of chemical modifications of oligonucleotides, applying antisense oligonucleotide-based strategies for the treatment of diseases has become possible. Libraries of vast numbers of oligonucleotide aptamers, microarrays of thousands of oligonucleotides on surfaces, and building block sequences for DNA nanostructures are also prepared by chemical synthesis. Common for all the oligonucleotides described above is that they are synthesized from nucleoside phosphoramidite building blocks[1].
High-quality phosphoramide and a good solid carrier are important factors for the successful synthesis of long oligos with artificially modified backbones, which could not be prepared easily by other methods.
The artificial analogs include alkyl/arylphosphonates, phosphotriesters, phosphoramidates, phosphorothioates, phosphorodithioates, phosphoroselenates and boranophosphates, which are attractive as antisense molecules. The phosphoramidite strategy has been and is being most widely employed in both liquid-phase and solid-phase syntheses of various kinds of oligos[2].
Fig. 1: Strategy for phosphoramidite synthesis in a flow-based setup[1].
Our Services:
With the commercialization of automated DNA synthesis technology, chemical oligonucleotide synthesis has become a tool in many research institutions. Creative Biolabs specializes in custom oligonucleotide synthesis. We have a catalog of several hundreds of available nucleoside and non-nucleoside phosphoramide building blocks ranging from fluorescent dyes and other dyes to protein ligands and redox labels.
As a manufacturer of complex oligonucleotides, we can produce a series of modified phosphoramide compounds, thoroughly purified, dried, and functionally tested, for oligonucleotide synthesis. And we can also provide customized phosphoramide compounds according to your needs. If any items you need are not included in the table below, please send us an inquiry.
Our phosphoramidite compounds include but are not limited to:
Advantages:
- Extensive experience
- Large-scale production
- High purity: we usually purify oligos by HPLC.
- Fast delivery: deliver within 2 weeks
- Competitive pricing
Creative Biolabs can supply professional customized services on chemical modifications of nucleic acid drugs and support your research and technology applications. Please contact us to learn more.
References
- Alexander F. Sandahl, Thuy J. D. Nguyen, Rikke A. Hansen, Martin B. Johansen, Troels Skrydstrup, Kurt V. Gothelf. On-demand synthesis of phosphoramidites. Nature Communications. 12,2760 (2021)
- Natarajan Venkatesan, Su Jeong Kim and Byeang Hyean Kim. Novel phosphoramidite building blocks in synthesis and applications toward modified oligonucleotides. Current Medicinal Chemistry. 10,1973-1991(2003)
*For Research Use Only. Not for use in diagnostic procedures.