Large-Scale DNA Oligonucleotide Synthesis
Oligonucleotides are small molecules of 8–50 nucleotides in length that bind via Watson-Crick base pairing to enhance or repress the expression of target RNA [1].
At present, synthetic oligonucleotides have been widely used in the fields of biotechnology, gene editing, molecular diagnosis and therapy. Natural oligonucleotides have been widely used in gene construction, DNA sequence selection and determination, and site-directed mutagenesis, etc. They are the basic reagents of PCR technology. In in vitro studies, they have also been used to selectively inhibit gene expression, and recently, their ability to interact with proteins has also been utilized.
Chemically modified oligos, such as the introduction of bases, sugar or phosphate groups, can expand the scope of nucleotide applications. However, due to chemical, electronic and spatial considerations, designing new modified oligonucleotides is a difficult task [2].
We can provide large-scale DNA synthesis and modification services to meet the needs of different applications.
About Creative Biolabs
We are one of the world's leading suppliers of synthetic oligonucleotides. High-quality production materials, as well as automated oligonucleotide synthesis and purification systems, allows us to produce high-quality oligonucleotides at competitive prices. We have carried out strict quality control at every step to remove impurities to ensure that you get the highest quality products.
Our Large-Scale DNA Oligonucleotide Synthesis Service
Thanks to the high-throughput oligosaccharide synthesis equipment, our production capacity is unparalleled. The range of our large-scale DNA synthesis, purified by high-performance liquid chromatography, and detected by HPLC, PAGE, MALDI-TOF or ESI/LC/mass spectrometry, can serve all customers whether they need a small or large amount of oligonucleotides.
Modifications and Probes |
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Backbone Modifications |
Linker Modifications |
qPCR Probes |
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Advantages of Our Services:
- A wide variety of modifications are available
- Fast and cost-effective
- Ships within 10 business days
- Quality ensured by 100% QC
Service Specifications:
- Purification options: Salt Free, HPSF, HPLC
- Scale: from 50 mg to multiple grams
- Unmodified DNA lengths between 4–100 bases
- Unmodified RNA lengths between 4–70 bases
Deliverables:
- Custom formulation and packaging
- lyophilized DNA powder
- COA file
QC Analysis:
- HPLC/LC-MS
- Endotoxin Control/Bioburden (optional)
References
- Ronak Lakhia,Vishal Patel. Manipulation of renal gene expression using oligonucleotides. Methods in Cell Biology 154, 109-120 (2019)
- M. GalloJ.M. MontserratA.M. Iribarren. Design and applications of modified oligonucleotides. Brazilian Journal of Medical and Biological Research. 36(2):143-51. (2003)
*For Research Use Only. Not for use in diagnostic procedures.