Large-Scale RNA Oligonucleotide Synthesis
RNA is an important biomolecule that exists in all biological systems and plays an important role in gene expression, regulation and development. It has become more important as an increasing number of functions, both regulatory and enzymatic, are being discovered on a daily basis. As the RNA boom has just begun, most techniques are still in development and changes occur frequently. To understand RNA functions, revealing the structure of RNA is of utmost importance, which requires synthesis and chemical modification[1].
Until a few years ago, the chemical synthesis of ribonucleic acid (RNA) was still in its infancy, far behind deoxyribonucleic acid (DNA) technology, and only a few groups in the world could successfully synthesize and purify it. However, with the recent intense interest in synthesizing RNA, as well as the development of ribozymes and aptamers for therapeutic and/or diagnostic applications, RNA synthesis has become routine and highly reliable. The boom in the use of synthetic RNA not only enables professional reagent manufacturers to improve the quality of their products, but also creates competition among suppliers, thereby reducing consumer costs[2].
Synthesis cycle of DNA and RNA
Our Large-Scale RNA Oligonucleotide Synthesis Services
Our final Oligonucletoides products are purified by HPLC/ PAGE and verified by independent QA (Quality Assurance) procedures with extremely low mutation and low error rates, which guarantee the highest quality large-scale RNA oligonucleotides. The products can be widely used in precision medicine, genetic disease testing, infectious disease testing, food, agriculture and other industries.
Available Modifications and Labeling
| m7GTP | 2' O-Methyl RNA bases | 5' -Biotin | 3' Amino Modifier | 5' 5-Methyl dC |
| Int 5-Methyl dC | 3' -Phosphorylation | Phosphorothioate Bond | 5' Amino Modifier C12 | FAM Labeling |
| Cy3 Labeling | Cy5 Labeling | Others |
Advantages of Our Services:
- A wide variety of modifications are available
- Fast and cost-effective
- Ships within 10 business days
- Quality ensured by 100% QC
Service Specifications:
- Purification options: Salt Free, HPSF, HPLC
- Scale: from 50 mg to multiple grams
- Unmodified RNA lengths between 4–70 bases
- Phosphorothioate (PS), Phosphodiester (PO) or PO/PS backbone
Deliverables:
- Custom formulation and packaging
- COA file
QC Analysis:
- HPLC/LC-MS
- Endotoxin Control/Bioburden (optional)
We provide high-quality and diversified RNA chemical synthesis services to produce RNA oligonucleotides at various quantities. Our customized RNA products, single-stranded/double-stranded RNA oligonucleotides, modified RNA oligosaccharides, stable siRNA, miRNA, long RNA, etc., have passed mass spectrometry and HPLC purity tests to ensure high quality. Please contact us for a free consultation.
References
- Lorenzo Baronti, Hampus Karlsson, Maja Marušič, Katja Petzold. A guide to large-scale RNA sample preparation. Analytical and Bioanalytical Chemistry 410, 3239-3252 (2018)
- Satoshi Obika·Mitsuo Sekine. Large-Scale Oligonucleotide Manufacturing. Synthesis of Therapeutic Oligonucleotides. 97-98(2018)
*For Research Use Only. Not for use in diagnostic procedures.