Locked Nucleic Acid Synthesis Services
Locked nucleic acid (LNA), as a special new bicyclic oligonucleotide derivative, is a modified RNA composed of six bases: A, C, G, T, U, and mC It has played a major role in the field of biology and pharmaceutical research. It has the advantages of high affinity, good thermal stability, strong resistance to enzyme cleavage, and non-toxicity in the body. It can be synthesized by solid-phase method like ordinary DNA primers. Therefore, it is widely used in many fields such as antisense therapy, single nucleotide polymorphism (SNP) detection and so on. The 2'-0,4'-C position of β-D-ribofuranose in the LNA structure forms a ring-shaped oxymethylene bridge, thiomethylene bridge or aminomethylene bridge through shrinkage, and the structure of furanose is locked in The N configuration of C3' endotype forms a rigid condensation structure. Based on the above-mentioned excellent performance of locked nucleic acid, its application in the design and synthesis of the following molecular beacons is expected to improve the performance of molecular beacons and become a new breakthrough in the treatment of various diseases at the molecular level.
Schematic diagram of locked nucleic acid structure
Creative Biolabs is a professional biotechnology services platform; provide outsourced experiments for oligonucleotide synthesis research. We have professional researchers and strict quality control to ensure the synthesis of locked nucleic acid.
- Design, synthesis and performance study of terminal modified locked nucleic acid molecular beacons.
- Design, synthesis and performance study of the modified locked nucleic acid molecular beacons of mismatch sites.
- Design, synthesis and performance research of mercury ion-specific locked nucleic acid molecular beacons.
Design principle of locked nucleic acid:
LNA will bind tightly to other LNA residues, self-complementation and cross-hybridization with other LNA containing longevity nucleotides should be avoided.
- GC content is maintained at 30-60%
- Avoid more than 4 LNA residues in a row, unless the designed oligonucleotide has only 9-10 nucleotides
- Avoid 3 or more consecutive G or C
Schematic diagram of locked nucleic acid
Synthesis method of locked nucleic acid:
- Solid phase method
- Aminophosphate process
Advantages of locked nucleic acid:
- High affinity
- Good thermal stability
- Strong resistance to enzyme cutting
- In vivo nontoxicity
Application range of locked nucleic acid:
- Antisense therapy
- Single nucleotide polymorphism (SNP) detection
- miRNA research
- Small RNA research
- Analysis of SNP Genotyping
- Synthesis of mRNA antisense oligonucleotides
- Allele specific PCR
- Fluorescence polarization probe
- Molecular beacon
- Microarray gene expression profiling
- Gene repair and exon skipping
- Splicing mutation detection
- Comparative Genome Hybridization (CGH)
References
- Yingzhou Tao, Weijia Wang, et al. Sensitive biosensor for p53 DNA sequence based on the photothermal effect of gold nanoparticles and the signal amplification of locked nucleic acid functionalized DNA walkers using a thermometer as readout. Talanta. 2020, 220.
- Haase Linn, Weisz Klaus. Locked nucleic acid building blocks as versatile tools for advanced G-quadruplex design. Nucleic Acids Research. Nucleic Acids Research.
*For Research Use Only. Not for use in diagnostic procedures.