RNA Chemical Modification Services
Why is RNA modification important?
Recently, significant interest has been observed in the use of modified oligoribonucleotides in the fields of molecular biology, biochemistry, and medicine. Substantial effort has been made towards the clinical application of RNA-based therapeutics, employing mostly antisense oligonucleotides and small interfering RNAs, with several gaining FDA approval. Selected RNA modifications have been proven to enhance the drug-like properties of RNA oligomers, providing the oligonucleotide-based therapeutic agents in the antisense and siRNA technologies. ;
The chemical synthesis of modified oligoribonucleotides also represents a powerful approach for studying the structure, stability, and biological activity of RNAs.
Introduction to RNA modifications
The representative RNA modifications include Phosphorothioate (modification to the 3' Phosphate), 2'-Fluoro or 2'-O-methyl (modifications to the ribose sugar), and Pseudouridine or 5-methylcytosine (modifications to the nitrogenous base).
The impact of the 2'-O-methyl groups alone is important because these modifications are present in modern RNA. These ribose modifications are known to increase the Tm values of RNA duplexes. An attractive way to prevent degradation from nucleases is the use of 2'F and 2'-O- methyl RNA bases, when specific 2'OH is not required. The 2'-O- methyl oligonucleotides confer considerable nuclease resistance and are similar in hydrogen bonding properties to RNA/RNA than the lower RNA/DNA binding property. The coupling efficiency of 2'-O- methyl phosphoramidite is also higher than the RNA monomers resulting in a higher yield of full-length oligos.
We offer a wide range of RNA modifications. We can also provide some special modifications (please contact us to inquire). Below is a list of the most frequently requested modifications.
5' -Modifications:
| 5'-TAMRA-hexyl linker | 5'-Amino,(6-carbon)linker | 5'-Phosphate | 5'-Amino,(12-carbon)linker | 5'Amino Modified C6 dT |
| Uni-Link Amino Modifier | 5'-Biotin | 5'-Photocleavable Biotin | 5'-Fluorescein | 5'-Tetrachloro-fluorescein |
| 5'-Pyrene | 5'-Thiol | 5'-Dabcyl | 5'-Cholesterol | 5'-Cy3TM |
| C9 Spacer | C18 Spacer |
3'-Modification
| 3'-Biotin | 3'-Inverted abasic | 3'-Inverted deoxythymidine | 3'-Puromycin | 3'Terminal dideoxy-cytidine |
| 3'-TAMRA | 3'-Fluorescein | 3'Terminal deoxy-guanosine | 3'-Cholesterol | 3'-Amino Modifier (6 atom) |
Internal Modifications
| 2,6-Diaminopurine | 2'-Amino-uridine | 2'-Deoxy-uridine | 2'-Fluoro-adenosine | 2'-Fluoro-cytidine |
| 2'-Fluoro-acetyl-cytidine | 2'-Fluoro-guanosine | 2'-Fluoro-uridine | 2-Aminopurine | 4-Thio-uridine |
| 5-Bromo-Uridine | 5-Fluoro-uridine | 5-Iodo-uridine | 5-Methyl-cytidine | 2'-Amino-cytidine |
| Inosine | N3-Methyl-uridine | Purine ribonucleoside | Ribo-thymidine | 5-Amino-allyl-uridine |
| Deoxy-abasic | N6, N6-Dimethyl-adenosine | C3 Spacer | C9 Spacer | C18 Spacer |
Degenerate Bases
| Mix site: rA, rG, rC and rU | Mix site: rA and rU | Mix site: rG and rC | Mix site: dA, dG, dC and dT | Mix site: dA and dT |
| Mix site: dC and dG | Mix site: mA, mG, mC and mU | |||
Advantages:
- Extensive experience
- Large scale: we can synthesize oligos on the 0.2 μmol, 1.0 μmol, 15 μmol and 30 μmol scales. ;
- High purity: we usually purify oligos by HPLC.
- Fast delivery: deliver within 2 weeks
- Special labels are available.
- Reports: We provide reports containing O.D. values, nmol, M.V. and TM of oligonucleotides.
- Competitive pricing
Creative Biolabs can supply professional custom services on chemical modifications of RNA oligos and support your research and technology applications. Please contact us to learn more.
Reference
- Steven Ochoa, Valeria T. Milam, Luigi A. Agrofoglio, ;Academic Editor, Vincent Roy, ;Academic Editor, and Cyril Nicolas, ;Academic Editor. Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides. Molecules.25(20): 4659. 2020
*For Research Use Only. Not for use in diagnostic procedures.