RNA Probe Synthesis Services
The RNA probe refers to a single-stranded cDNA or cRNA molecule with a label that can complementarily bind to the corresponding nucleotide sequence in the tissue. As a kind of promising nucleic acid probes, RNA probes are mainly used for research purposes. RNA probe detection is fast, simple and sensitive.
As an important nucleic acid probe, RNA probe has been widely used in biomedical field: molecular biology research, research on genetic disease genes, genetic diagnosis, molecular in situ hybridization, virus identification test, diagnosis of infectious diseases and tumor early screening research.
Creative Biolabs is a professional bio-technology services platform providing outsourced oligonucleotide synthesis services. We have professional researchers and strict quality control, can provide there types of the following RNA probe synthesis.
The types of RNA probes:
|cRNA probe||Single-stranded cDNA probe||Oligonucleotide probe|
Synthesis method of RNA probe:
- Enzymatic reaction method
- End labeling method: It is a labeling method to introduce the label into the 5 or 3 ends of linear DNA or RNA. The end labeling is suitable for the labeling of synthetic oligonucleotide probes. The probes prepared with the end labeling are generally carried There are fewer marker molecules.
- In vitro transcription: It is a method of preparing single-stranded RNA probes with the same sequence as the fragment.
- Chemical reaction method
In vitro transcription synthesis flow chart
Chemical synthesis is a process of synthesizing RNA probe by using compounds as templates, PCR and other biological means combined with RNA probe transcription in vitro.
- Golab Karolina, Krzystyniak Adam, et al. Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization Biology&Molecular Biology. 2020.
- Thomas J. X. Li, et al. On an enhancement of RNA probing data using information theory. Algorithms for Molecular Biology. 2020, 15(1): 167-212.
- Dresselhaus Thomas, Bleckmann. Andrea Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization. Methods in molecular biology. 2020, 2166: 3-21.
*For Research Use Only. Not for use in diagnostic procedures.