siRNA Synthesis Services

The small interfering RNA (siRNA) is a 20 to 25 nucleotide long double stranded RNA (dsRNA). siRNA specifically degrades complementary target mRNA through activation to inhibit target protein expression. siRNA synthesis is the simplest way to achieve gene silencing in the organism or cell with low cost and high transfection efficiency, and it is also the best method for RNAi clinical therapy, which has irreplaceable advantages in drug development. Conventional chemically synthesized siRNA products are purified by HPLC to completely remove unpaired single strands. We can provide conventional siRNAs as well as a variety of modified siRNAs.

Mechanism of action of siRNA

Creative Biolabs is a professional biotechnology services platform, provide outsourced experiments for oligonucleotide synthesis research. We have professional researchers and strict quality control to ensure the synthesis of high-quality siRNA.

Applications of siRNA synthesis:

• Gene knockout
• Transgenic animals
• Functional genomics and other studies
• Gene therapy
• Drug research

The construction process of siRNA vector

Methods of siRNA synthesis:

Chemical synthesis of siRNA molecules	Features: High cost, but convenient to provide high quality chemical synthesis siRNA directly according to user requirements. Most applicable: When the most effective siRNA has been found, a large number of siRNA studies are required.
In vitro transcription of siRNA molecules	Characteristics: The cost is lower than that of chemical synthesis method. it is a good method of screening siRNAs with higher cost performance. The obtained siRNAs can achieve the effect of chemical synthesis of siRNAs with high concentration as long as the concentration is low. Advantages: The siRNA obtained from in vitro transcription has low toxicity, good stability and high efficiency. To achieve the same transfection efficiency requires only 1/10 of the amount of chemical synthesis. Most suitable for: Screening siRNAs, especially preparation of multiple siRNAs.
Long fragments of dsRNA were digested by RNAS II to prepare siRNA	Characteristics: Select the target mRNA template of 200-1000 bases, and long fragments of double-stranded dsRNA are prepared by in vitro transcription, and then digested by Ranse III (or Dicer) in vitro, many siRNAs are obtained. After removing the undigested dsRNA, the siRNA mixture can be directly transfected into the cell, in the same way as single siRNA transfection. It is usually possible to ensure that the target gene is effectively suppressed. Advantages: high efficiency; Can skip the detection and screening of effective siRNA sequence steps; save time and money. Best for: rapid and economical study of the phenotype of a gene's functional loss.
SiRNA expression vector	Characteristics: The expression vector continuously produces siRNA through transcription in cells, it can prolong the action time of siRNA. Advantages: It can be studied for a long time, and the carrier with antibiotic marker can suppress the expression of target gene continuously in the cell. Most applicable: a valid siRNA sequence is known, gene silencing needs to be maintained for a long time, or antibiotics need to be used to screen cells that can express siRNA for long-term studies.
SiRNA expression framework	Characteristics: siRNA expression Cassettes (SECS) is a template of siRNA expression obtained by PCR; It can be directly introduced into cells for expression. SECS is the most effective tool for siRNA screening, and even the best combination of promoter and siRNA can be screened in a specific research system. Alternatively, the most effective siRNA screened by SECS can be directly cloned into the vector to construct the siRNA expression vector. Advantages: No need for vector cloning and sequencing, it is the most effective siRNA screening tool, even can be used to screen the promoter and siRNA in a specific research system is the best match. Most suitable for: siRNA sequence screening, screening the best promoter before cloning to the vector.

SiRNA technology process and application

Conventional siRNA synthesis (15-25bp) dual stranded

Modified siRNA synthesis (15-25bp) dual stranded

In addition to the above modifications, if you need other modifications please contact us.

Competitive Advantages:

Flexible synthesis scales
Bulk orders are available upon request.
Stringent quality control
High-quality and cost-effective
Both unmodified and modified RNA oligos are available.
Comprehensive backbones
Fast turnaround: 5-10 business days

Quality Assurance

Complete order service system
All RNA oligonucleotides are manufactured under strict SOP quality control process
High resolution Waters chromatography with ESI-MS to ensure purity

Delivered results

Synthetic RNA lyophilized powder
COA documentation
Assay reports (MS & HPLC)

References

1. Wu S Y, Romain Guyot, et al. Structural and Functional Annotation of Transposable Elements Revealed a Potential Regulation of Genes Involved in Rubber Biosynthesis by TE-Derived siRNA Interference in Hevea brasiliensis. International Journal of Molecular science. 2020, 21.
2. Mieke F. van Essen, Nicole Schlagwein, et al. Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells. Journal of Immunological Methods. 2020,479.

*For Research Use Only. Not for use in diagnostic procedures.

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