Long DNA Oligo Synthesis Services
The chemical synthesis of long DNA oligonucleotides is the foundation of synthetic biology. It was widely used in diverse fields, including codon optimization and in vitro functional evaluation of genes, nucleic acid immunity and gene chip preparation, etc[1].
Applications of DNA long-chain oligonucleotides include but are not limited to:
- Cloning, gene construction and site-directed mutagenesis
- shRNA library preparation
- RNA Synthesize in vitro transcription
- PCR Assay
- SNP Analysis
- Sequencing
Standard DNA synthesis
Standard DNA synthesis, including the most advanced silicon chip-based platform technology, still relies on phosphoramide chemical synthesis methods[2]. Phosphoramidite-based oligo synthesis most commonly consists of a four-step cycle that adds bases one at a time to a growing oligo chain attached to a solid support[3].
The four-step synthetic oligo synthesis is the most commonly used chemistry for the production of DNA oligos[4].
The limitations of this 40-year-old technology are obvious. In the early days, molecular biology relied mainly on short DNA sequences, for example as PCR primers or probes. Researchers are now looking for longer oligonucleotides to meet the growing demands of biopharmaceuticals and high-complexity synthetic biology. But phosphoramidite-based oligo synthesis cannot provide long nucleotide DNA (>150 nt) of sufficient quality and quantity to meet the needs of biopharmaceutical and other fields[4].
Our Long DNA Oligo Synthesis Services
Creative Biolabs has developed a proprietary method to manufacture DNA oligonucleotides of up to 300 bases with an industry-leading low error rate. Our optimized engineering solutions utilize advanced equipment and a strong technical team to support our long oligonucleotide synthetic services and prevent errors, including deletions, substitutions, insertions and fragmentation, which typically compound with longer DNA fragments.
Advantages:
- Up to 300 bases
- Normalized and purified in various scales
- High Throughput
- Superior quality and yield
- Competitive pricing
- Reliable service and fast turnaround time
- Modified and unmodified oligos are available.
Purification
When the length of oligos increases, there are more deletions in the oligonucleotide sequence. Therefore, we strongly recommend using polyacrylamide gel electrophoresis(PAGE) to purify oligonucleotides of more than 50 bases.
Purified by PAGE, its purity can reach 97%~99.9%.
Available Modifications
| 5' deoxyInosine | 5' deoxyUridine | 5' Phosphorylation | Int deoxyInosine | Int deoxyUridine |
| 2' O-Methyl RNA bases | 5'-Biotin | 3' Ribo A | 3' Ribo C | 3' Ribo G |
| 3' Ribo U | 3' Amino Modifier | 5' 5-Methyl dC | 5' 5-Methyl dC | Int 5-Methyl dC |
| 3' -Phosphorylation | Int Spacer 18 | Phosphorothioate Bond | 5' Amino Modifier C6 | 5' Amino Modifier C12 |
Delivery:
- Synthetic DNA
- COA
- Test report (MS & HPLC)
Creative Biolabs is the world's leading supplier of customized oligo products and services. We have a leading nucleic acid synthesis, purification and analysis platform and professional talents to provide high-quality and reliable cGMP-level and non-cGMP-level oligonucleotide products. For many years, we have been committed to providing high-quality oligonucleotide synthesis and formulation services for global partners.
References
- Subha SankarPaula, HeykelTrabelsi, YazenYaseen, UpasanaBasu, Hiyam AdilAltaii, DebarunDhali. Advances in long DNA synthesis. Microbial Cell Factories Engineering for Production of Biomolecules. 21-36 (2021)
- Michael Eisenstein. Enzymatic DNA synthesis enters new phase. Nature Biotechnology. 38, 1113-1115 (2020)
- Sriram Kosuri,George M Church. Large-scale de novo DNA synthesis: technologies and applications.Nature Methods. 11, 499-507 (2014)
- Yang J-K, Chen F-Y, Yan X-X, Miao L-H, Dai J-H (2012) A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia. PLoS ONE 7(5): e36607.(2012)
*For Research Use Only. Not for use in diagnostic procedures.