Long Oligo Synthesis Services
Long oligonucleotides are very useful in drug discovery and development, and data storage. In addition, long oligonucleotides can provide support for CRISPR gene editing and protein engineering applications, where researchers can greatly increase their productivity.
Long oligonucleotide synthesis, or the chemical synthesis of long nucleic acids, has become an important tool in the field of molecular biology. Synthetic long oligonucleotides have been utilized in numerous research applications, such as diagnosis of genetic and infectious diseases, new drug discovery, and disease treatment[1].
Limitations of Conventional Oligo Synthesis
In the past decades, the chemical synthesis of nucleic acids has overwhelmingly been performed using variations of the phosphoramidite chemistry on solid surfaces. The reasons for this universal adoption are both the efficiency of the chemistry and its versatility resulting in numerous adaptations to achieve specific needs.
However, although there has been significant demand for libraries of long oligos (150mer and more), the yields in conventional oligo synthesis and the associated side reactions have previously limited the availability of oligonucleotide pools to lengths <100 nt. As the oligonucleotide length starts to exceed 100 nucleobases, these limitations have larger effects. A successful synthesis of long oligonucleotides requires a stepwise coupling yield of ≥ 99.5%.
Our Long Oligo Synthesis Services
Breaking through limitations, Creative Biolabs has developed a proprietary method to manufacture oligonucleotides of up to 300 bases with an industry-leading low error rate. Our optimized engineering solutions utilize advanced equipment and a strong technical team to support our long oligonucleotide synthetic services and prevent errors, including deletions, substitutions, insertions and fragmentation, which typically compound with longer DNA fragments.
Advantages:
- Superior quality and yield: highest coupling efficiency of ≥ 99.5%, lowest error rates of typically 0.09 %, and best cloning results with > 85 % perfect clones.
- Unmatched synthesis length: DNA of up to 300 bases and RNA synthesis up to 120 bases.
- Competitive pricing
- Reliable service and fast turnaround time
- Modified and unmodified oligos are available.
Purification
When the length of oligos increases, there are more deletions in the oligonucleotide sequence. Therefore, we strongly recommend using polyacrylamide gel electrophoresis(PAGE) to purify oligonucleotides of more than 50 bases.
Purified by PAGE, its purity can reach 97%~99.9%.
Available Modifications
| 5' deoxyInosine | 5' deoxyUridine | 5' Phosphorylation | Int deoxyInosine | Int deoxyUridine |
| 2' O-Methyl RNA bases | 5'-Biotin | 3' Ribo A | 3' Ribo C | 3' Ribo G |
| 3' Ribo U | 3' Amino Modifier | 5' 5-Methyl dC | 5' 5-Methyl dC | Int 5-Methyl dC |
| 3' -Phosphorylation | Int Spacer 18 | Phosphorothioate Bond | 5' Amino Modifier C6 | 5' Amino Modifier C12 |
References
- Sumate Pengpumkiat,Myra Koesdjojo,Erik R. Rowley,Todd C. Mockler,Vincent T. Remcho.Rapid Synthesis of a Long Double-Stranded Oligonucleotide from a Single-Stranded Nucleotide Using Magnetic Beads and an Oligo Library.PLoS ONE. 11(3): e0149774. (2016)
- Emily M. LeProust,Bill J. Peck,Konstantin Spirin,Heather Brummel McCuen,Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process.Nucleic Acids Res.38(8): 2522–2540.(2010)
*For Research Use Only. Not for use in diagnostic procedures.