Single-Stranded DNA Synthesis Services
Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology[1]. Applications of ssDNA oligos include but not limited to the development of novel clinical applications, isogenic disease models generation, CRISPR gene therapy, DNA nanotechnology, in vitro transcription, etc.
Efficient ssDNA synthesis is a major need for numerous biotechnology applications including templated homology-directed repair for genome editing, systems-scale gene synthesis and cloning, and scaffolded DNA origami. Among them, the applied research in CRISPR/Cas9 technology is very popular.
Advantages of ssDNA synthesis for CRISPR/Cas9 technology:
- Lower cellular toxicity compared to dsDNA
- Low off-target integration
- High specificity knock-in templates
- High efficiency donors
- High editing efficiency
- Increased editing accuracy
Conventional ssDNA synthesis is performed using either chemical or enzymatic approaches. Chemical synthesis is currently limited to approximately 98% incorporation efficiency for each base addition and is therefore limited to the production of ssDNA oligonucleotides up to only 200 bases. Subsequent enzymatic synthesis through ligation or polymerization yields double-stranded DNA (dsDNA), enabling complete gene synthesis that can be further parallelized, but requires additional steps to generate ssDNA. Alternatively, direct synthesis of longer ssDNA from oligonucleotides can be accomplished by primer exchange reaction[2].
Our Long ssDNA Synthesis Services
Long ssDNA sequences are difficult to produce in the lab, especially at high concentrations necessary for gene editing experiments. But based on our extensive experience in oligo synthesis and our powerful synthesis platform, Creative Biolabs is able to successfully provide high-quality ssDNA modification and synthesis services according to specific requirements of our customers.
Nowadays, the conventional chemical synthesis method of ssDNA oligonucleotides is solid-phase DNA synthesis. However, as the length of oligos increases by the chemical method, the output will decrease. Therefore, in current chemical research, it is not effective to synthesize long, high-quality DNA by chemical methods. We have developed an enzymatic synthesis method to produce long ssDNA of up to 400 bases with an industry-leading low error rate.
Advantages of Our Services:
- Modifications available
- Fast and more cost-effective
- Deliver in 4 weeks
- Up to 20 µg delivery quantity
Deliver:
- 2, 3, 6, 10, 20, or 40 µg lyophilized DNA
- The final ssDNA product Sanger Verified by sequencing
- Size verification by gel electrophoresis
- S1 nuclease digestion test
- COA
Creative Biolabs is the world's leading supplier of ssDNA and dsDNA products and services. We have a leading nucleic acid synthesis, purification and analysis platform and professional talents to provide high-quality and reliable cGMP-level and non-cGMP-level oligonucleotide products. For many years, we have been committed to providing high-quality oligonucleotide synthesis and formulation services for global partners.
References
- Tulsi Ram Damase,Andrew D. Ellington,Peter B. Allen.Purification of single-stranded DNA by co-polymerization with acrylamide and electrophoresis.BIOTECHNIQUES. 62, 6 (2018)
- Rémi Veneziano,Tyson R. Shepherd,Sakul Ratanalert,Leila Bellou,Chaoqun Tao,Mark Bathe.In vitro synthesis of gene-length single-stranded DNA.
- Scientific Reports.8, Article number: 6548 (2018)
*For Research Use Only. Not for use in diagnostic procedures.