Long RNA Oligo Synthesis Services
Synthetic RNA occupies a very special place in modern research. Custom solid-phase synthesis of 20-30 nucleotide-long strands has become a powerful driving force in many fields of biochemical and pharmaceutical research. Discoveries of the twenty-first century created a strong need for a robust solid-phase synthesis of longer RNA strands, over 100 nucleotides (-nt) in length[1]. Long RNA oligonucleotides are increasingly important in genomic function studies, drug discovery research and biosynthesis. In recent years, with the remarkable progress of RNA-related research, the demand for long-chain RNA is increasing. However, the chemical synthesis and purification of long-chain RNA (>50mer) face unique challenges due to their sequence composition, secondary structure and modifications.
Although several ingenious strategies have been developed to minimize these problems and to improve the synthesis of long RNA, the chemical complexity of solid-phrase RNA synthesis dictates that constructs longer than 50 nucleotides in length remain difficult to prepare. Most biologically important RNA molecules such as ribozymes, aptamers, and riboswitches are significantly longer than this, so new approaches to synthesize RNA analogs are urgently required.
Although RNA synthesis by transcription might seem a viable alternative, it does not allow the site-specific incorporation of multiple modifications at sugars, bases, or phosphates. In contrast, automated solid-phase RNA synthesis is compatible with the introduction of fluorescent tags, isotopic labels (for NMR studies) and other groups to improve biological activity and resistance to enzymatic degradation. The scope and utility of important RNA constructs can be significantly extended by such chemical modifications, and the scale of chemical synthesis is potentially unlimited. These are important factors when planning structural studies on RNA or the development of therapeutic oligoribonucleotides analogs, and should be considered when devising new methods of RNA assembly[2].
Applications of Long RNA Oligos:
- Single-guide RNA for CRISPR genome editing
- RNA-based diagnostics and therapeutics development
- Learn about the effects of RNA modifications on RNA-protein complexes
Our Long RNA Oligos（RNA Longmers）Synthesis Services
Based on our extensive experience in oligo synthesis and our powerful synthesis platform, Creative Biolabs can provide high-quality long RNA modification/labeling and synthesis services according to the specific requirements of our customers. We can produce long-chain RNA oligonucleotides of up to 200 bases with an industry-leading low error rate.
Advantages:
- Up to 200 bases
- High yield from ug to mg
- High Throughput
- Superior quality
- Deliver normalized and lyophilized
- Competitive pricing
- Reliable service and fast turnaround time.
Ships in 8 business days
Purification
When the length of oligos increases, there are more deletions in the oligonucleotide sequence. Therefore, we strongly recommend using polyacrylamide gel electrophoresis(PAGE) to purify oligonucleotides of more than 50 bases.
Purified by PAGE, its purity can reach 97%~99.9%.
Available Modifications and Labeling
| m7GTP | 2'O-Methyl RNA bases | 5'-Biotin | 3'Amino Modifier | 5' 5-Methyl dC |
| Int 5-Methyl dC | 3' -Phosphorylation | Phosphorothioate Bond | 5'Amino Modifier C12 | FAM Labeling |
| Cy3 Labeling | Cy5 Labeling | Others |
Creative Biolabs is the world's leading supplier of customized oligo products and services. We have a leading nucleic acid synthesis, purification and analysis platform and professional talents to provide high-quality and reliable cGMP-level and non-cGMP-level oligonucleotide products. For many years, we have been committed to providing high-quality oligonucleotide synthesis and formulation services for global partners.
References
- Muhan He, Xunshen Wu, Phensinee Haruehanroengra, Irfan Khan, Jia Sheng, Maksim Royzen.Bio-Orthogonal Chemistry Enables Solid Phase Synthesis of Long RNA Oligonucleotides. Chemical Communications. (2020)
- Afaf H. El-Sagheer,Tom Brown.New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes.PNAS. 107 (35) 15329-15334 (2010 )
*For Research Use Only. Not for use in diagnostic procedures.