Small Angle X-Ray Scattering
Small angle X-ray scattering is an important research method of biological structure. When X-rays are irradiated on the sample, if there are Nano-scale electron density uneven areas inside the sample, they will appear in a small angle range around the incident beam (generally 2θ≤5%) scatter X-rays. Small-angle X-ray scattering is particularly sensitive to the inhomogeneity of electron density. Any material with Nano-scale electron density inhomogeneity will cause small-angle scattering.
Small angle X-ray scatterometer (Catherine Cheu, Lin Yang; et al, 2020)
Creative Biolabs is a professional biotechnology services platform, provide pre-clinical research outsourcing services. Operating in accordance with global regulations and fully accredited by AAALAC, Creative Biolabs ensure the overall health and safety of laboratories and save time and money to our customers. Our researchers are all from accredited organizations and institutions and are rigorously trained to ensure that the quality of small angle X-ray scattering technical services meets national standards.
Small-angle X-ray scattering research object:
Bio-macromolecules: such as proteins, nucleic acids, viruses, peptides, etc.
Schematic diagram of small-angle x-ray scattering principle
We provide but are not limited to:
- Qualitative analysis of the structure of biological macromolecules
- Identify morphological changes of biological macromolecules
- Quantitative study of the long program size of biological macromolecule samples
- Analysis of the long-range law of protein and its complex sample parameters
- Determination of molecular parameters of biological macromolecules
- Analyze the conformation of biological macromolecules
Advantages of small angle X-ray scattering:
- Simple sample preparation
- No need determine the tightness inside the sample
- Simultaneous research on living or dynamic biological macromolecule samples
- Obtain strong scattering signals of proteins and their complexes
- High cost performance
- International leading talent team
- Strictly comply with international standards and rules
- A fully integrated experimental platform
Sample preparation requirements:
- Powder sample: more than 2 mg, preferably more than 10 mg
- Liquid sample: concentration higher than 10mg/mL, volume more than 1 mL; must be placed in a transparent container (such as a capillary tube) for testing. Solute is completely dissolved in solvent, that is, there is no precipitation. The difference in electron density between solute and solvent should be as large as possible
- Highly toxic sample: specially specified
- The storage conditions of each sample, such as routine, freezing, drying, cold storage, light protection, etc., should be specified
References
- Krzysko Anthony J, et al. Correlating inter-particle forces and particle shape to shear-induced aggregation/fragmentation and rheology for dilute anisotropic particle suspensions: A complementary study via capillary rheometry and in-situ small and ultra-small angle X-ray scattering. Journal of colloid and interface science. 2020, 47-56.
- Catherine Cheu, Lin Yang, et al. Refining Internal Bilayer Structure of Bicelles Resolved by Extended- q Small Angle X-Ray Scattering. Chemistry and Physics of Lipids. 2020.
*For Research Use Only. Not for use in diagnostic procedures.